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We have carried out genome-wide imputation from 60 CEU HapMap2release 22NCBI build 36reference panels18using MACH 1.0.1656resulting in 2,588,592 autosomal HapMap-based SNPs2,168,668 SNPs fulfilled the quality criteriawhich are as mentioned above for genotyped SNPs and additionally consisted of an imputation-r 20.5.
The discovery population and the design used to determine AAT-associated genetic variants are depicted in Figure S1 and further described in the Materials and Methods sectionA comparison between the characteristics of the genome-wide analyzed sampleSAPALDIA discovery armN1392and the remainder of the SAPALDIA cohortSAPALDIA replication armN4245did not reveal substantial obat zovirax. differences in AAT serum levels or covariate distributionTable S1although asthmatics were overrepresented39.4in the SAPALDIA discovery arm and absent in the replication armwhich is due to previous study design17The participants of the independent replication cohort CopenhagenN8273were on average five years older and had twice as many current smokersTable S1This was in line with substantially lower lung function levelsmore than 800 mL lower forced expiratory volume in 1 secondFEV1compared to both SAPALDIA subsetsand slightly elevated AAT blood levels1.339 g/L vs1.257 and 1.255 g/LrespectivelyThe characteristics of the study populations contributing to the genetic association analyses with lung function are given in Table S2.
For the replicated SNP in the SAPALDIA replication armas well as for the lung function analysisa two-sided p-value of 0.05 was considered significantWe investigated heterogeneity between asthmatics and non-asthmatics in the discovery arm by testing for a difference between the two effectsusing a chi-square test with one degree of freedom.
AffiliationsSwiss Tropical and Public Health InstituteBaselSwitzerlandUniversity of legal viagra alternative. BaselBaselSwitzerland.
AffiliationDepartment of Pathology and Medical BiologyUniversity Medical Center GroningenGRIAC Research InstituteUniversity of GroningenGroningenThe Netherlands.
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